![]() (2017) Mechanism of dual-targeting of the phytochrome signaling component HEMERA/pTAC12 to plastids and the nucleus. – Nevarez P.A., Qiu, Y., Inoue, H., Yoo, C., Benfey, P.N., Schnell, D.J., Chen, M. – Yang, E.M., Yoo, C.Y., Liu, J., Wang, H., Cao, J., Li, F-W, Pryer, K.M., Sun, T., Weigel, D., Zhou, P.*, Chen, M.* (2019) NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches. (2020) Nucleus-to-plastid phytochrome signalling in controlling chloroplast biogenesis. – Qiu, Y.*, Pasoreck, E.K., Yoo, C.Y., He, J., Wang, H., Li, M., Larsen, H.D., Cheung, S., Chen, M.* (2021) RCB initiates Arabidopsis thermomorphogenesis by stabilizing the thermoregulator PIF4 in the daytime. (2021) Direct photoresponsive inhibition of a p53-like transcriptional activation domain in PIF3 by Arabidopsis phytochrome B. – Yoo, C.Y., He, J., Sang, Q., Qiu, Y., Long, L., Kim, R.J., Chong, E., Hahm, J., Morffy, N., Zhou, P., Strader, L.C., Nagatani, A., Mo, B., Chen, X., Chen, M. We currently focus on elucidating the mechanism of early temperature signaling in the control of temperature-responsive genes. We showed that phytochrome B (phyB) mediates daytime temperature sensing in Arabidopsis via temperature-responsive photobody dynamics as well as HMR- and RCB-dependent stabilization of the central thermal regulator PIF4. In Arabidopsis, a shift in ambient growth temperature of only a few degrees can significantly alter the expression of hundreds of temperature-responsive genes, resulting in dramatic adaptive responses in plant development, growth, metabolism, and immunity these responses are collectively referred to as thermomorphogenesis. Warm temperatures usually coincide with high light conditions during the daytime. Increases in global temperature are expected to drastically reduce crop productivity, understanding the mechanism of temperature signaling has become imminent to create a knowledge base for devising strategies to sustain crop production on a warming planet. Light signal transduction in higher plants. Arabidopsis HEMERA/pTAC12 initiates photomorphogenesis by phytochromes. Trends Cell Biol 21(11):664-71 (* Corresponding author). Phytochrome signaling mechanisms and the control of plant development. – Van Buskirk E.K., Decker, P.V., Chen, M. (2017) Mechanism of early light signaling by the carboxy–terminal output module of Arabidopsis phytochrome B. – Qiu, Y., Pasoreck, E.K., Reddy, A.K., Nagatani, A., Ma, W., Chory, J., Chen, M. (2019) Daytime temperature is sensed by phytochrome B in Arabidopsis through a transcriptional activator HEMERA. (2019) Phytochrome activates the plastid-encoded RNA polymerase for chloroplast biogenesis via nucleus-to-plastid signaling. – Yoo, C.Y., Pasoreck, E.K., Wang, H., Cao, J., Blaha, G.M., Weigel, D., Chen, M. (2020) Increasing ambient temperature progressively disassembles Arabidopsis phytochrome B from individual photobodies with distinct thermostabilities. These breakthroughs opened a new avenue to elucidate the mechanism of light and temperature signaling in plants and established photobody as a genetic experimental model to understand the general principles of subnuclear biomolecular condensates in cell signaling and transcriptional regulation. Our molecular genetic studies link photobodies to the regulation of the stability and activity of a group of nodal transcriptional regulators called Phytochrome-Interacting Factors (PIFs). Using forward genetic screens, we identified three novel phyB signaling components required for photobody formation, including HEMERA, RCB, and NCP. ![]() We showed that photobody localization is mediated by phyB’s C-terminal signal-output module, specifically by the dimerization/oligomerization of the histidine-kinase-like domain. ![]() Accomplishments include the determination of intrinsic phyB domains as well as extrinsic factors required for photobody formation. We aim to elucidate the mechanisms of the formation, function, and regulation of photobodies in phyB signaling and the environmental control of gene expression. We study how the plant photoreceptor and thermosensor phytochrome B (phyB) controls gene expression through dynamic assembly and disassembly of the phyB-containing subnuclear membraneless organelles or biomolecular condensates, named photobodies.
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